Oklahoma State University
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The GPC Microarray Core maintains state-of-the-art instrumentation for microarray production and analysis

 

 

OmniGrid100:Omni Grid The OmniGrid 100 microarrayer can print biological samples on standard sized glass slides, 100 slides at a time. Printing is from microtiter plates with 96 or 384 wells. A gripper arm allows up to 72 source plates to be used with full walk-away automation of 22,000 samples. The positioning system is high speed and uses high-reliability brushless DC servo motors. The instrument is enclosed in a humidity controlled chamber. The resolution is 2.5 micons along the X and Y axes and 1.25 microns along the Z axis. Thanks to the funding from the Dean's office, our new Telechem 946 printing pins can print features as small as 60-microns. That means we can get over 100,000 probes arrayed per microscope slide!  That's 10-million probes arrayed in a single print run taking only about 10-hours.   Check our Facility Fees and bring your genome to us to be printed at a VERY reasonable cost!

 

The NanoDrop II from Innovadyne Technologies, Inc is a non-contact nanoliter liquid dispensing instrument recently acquired by OMCF to provide additional capabilities. This instrument an accurately dispense volumes down to 25 nanoliters onto any surface, using eight solenoid ink-jets. A key technology for this instrument is that it uses an aspirate-dispense mode where the samples never contact the ink-jet valves. Th is creates a highly reliable, robust, and precise dispensing system. In addition, samples dispensed using this instrument are never heated, or subjected to severe shearing forces as with other small-volume dispensing systems. The NanoDrop II can dispense not only nucleic acids, but can dispense proteins in native form, derivitized beads, or even live bacterial or animal cells.  The software also provides for these biomolecules to be printed into arrays within the wells of 96-well plates. This opens up a complete new range of array technologies including protein-interaction studies, kinetic assays, and bead-based interactions or sensors. The ability to dispense onto fragile surfaces like nanofibers, hydrogels, MEMS devices, or channel glass are also opening up new collaborative efforts. Finally, the NanoDrop is designed to perform high-throughput micro-protein crystallography, and high-through microassays.

 

 

NanoDrop ND-1000(s): ND-1000We have two of these extremely popular and sensitive spectrophotometers to measure nucleic acid (DNA and RNA) and protein concentrations, calculate microarray fluorescent dye concentrations and determine cell densities. The nanoDrop 1000 produces a full spectrum analysis from   1-2 microliters of undiluted samples, and is completed in 10 seconds.

 

 

 

NanoDrop ND-3300: The NanoDrop 3300, is an extraordinary and versatile fluorospectrophotometer capable of accurate fluorometric determinations of nucleic acids (DNA, dsDNA, and RNA) and proteins using only 1-2 microliters of undiluted samples. ND-3300

From the Website: "The excitation source comes from one of the three solid-state LEDs housed in a block adjacent to the measurement pedestal (see below for more details about the LEDs). Emitted light is collected using the traditional 90° angle from the respective excitation source. A 2048-element CCD array detector, covering 400-750nm, is connected by an optical fiber to the optical measurement surface. The spectrometer is configured with a cut filter to eliminate light transmission below 395 nm."  A full spectrum of emission wavelengths is generated within 10 seconds.

 

Scanners:

 

Axon GenePix® 4400A 4-laser scanner from MDS Analytical Technologies
Our Axon GenePix 4400A 4-laser confocal  scanner was purchased through the generosity of the Vice President of research Core Facility Infrastructure Program. This exceptional instrument has increased speed and resolution for microarray scanning. It also allows the use of four different fluors simultaneously. The four-laser setup allows you to perform four conditions simultaneously. The 4-laser capability can be used for many types of SNP-analyses, and by quenching of molecular beacons, a process known as "FRET" can be performed using on-chip interactions.

 

 

 

 

ScanArray Express: Scan Array ExpressThe ScanArray Express is a dual wavelength ("red" & "green") scanner that supports over 50 fluorescent dyes.  It is used to quantify gene expression, genotyping and SNP analysis. With the arrival of the new Axon scanner, this workhorse will still be used, and will be important for the microarray workshops. This instrument was broken for a while, but with patience and effort we have made great progress in bringing this workhorse back to life. It's still a good scanner, and the ScanArray software is good too!

 

 

 

Beckman Biomek 2000: Biomek 2000To help out with repetitive pipettng tasks and manipulation of libraries that accompanies microarray production, a Beckman Biomek 2000 automation station is available to researchers. Normalization Wizard software is also available to generate a BioWorks method to automatically generate concentration-normalized copies of DNA sample plates.

 

 

 


ABI 7300 Real-Time PCR System: RT_PCRThis system combines thermal cycling, fluorescence detection, and application-specific software to provide an integrated platform for the detection and quantification of nucleic acid sequences. Real-time PCR reactions run in 25–100 µL volumes using 96-well plates, individual tubes or 8 tube strips and can be completed in under two hours. Four-color detection provides enables a variety of applications, including precise measurement of changes in gene expression, pathogen quantitation, SNP genotyping, and plus/minus assays that utilize internal positive controls.